A synthetic single-plate ELISA dataset with one antigen measured on a standard 96-well plate. Intended as a minimal worked example for users coming from a plate-reader workflow.
Format
A named list with the following elements:
- standards
Data frame of calibration standards. Columns:
curve_id(integer),dilution(numeric),od(numeric, raw optical density).- samples
Data frame of test samples. Columns:
curve_id,sampleid,dilution,od.- blanks
Data frame of blank-well readings, or
NULL. Columns:curve_id,od.- response_var
Character. Name of the response column (
"od").- indep_var
Character. Name of the independent variable column (
"dilution").- curve_id_lookup
Data frame with columns
curve_idandlabel.
Details
OD values are simulated on an eight-point dilution series.
The dataset is kept deliberately simple — one antigen, one plate —
so that the full preprocessing-to-back-calculation pipeline can be
demonstrated without the multi-plate complexity of
bead_assay_example.